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Transcription factors (TFs) are essential for the regulation of gene expression and are found in all living organisms. P2TF is an integrated and comprehensive database of TF proteins, which contains a compilation of the TF genes within completely sequenced genomes and metagenomes. It provides detailed annotation of each TF gene including classification, sequence features, functional domains, as well as chromosomal localization. Data can be downloaded for analysis and users can validate and add annotation to predicted TF genes.